Discrimination of Peromyscus maniculatus Leukocytes by Flow Cytometry
نویسندگان
چکیده
Deer mice (Peromyscus maniculatus) have been identified as the principal host species for Sin Nombre hantavirus. Assessment of deer mouse immune responses to this agent has been difficult because of a lack of reagents with defined specificity for the discrimination of leukocyte populations. The purpose of this work was to identify currently available immunological reagents directed at house mouse (Mus musculus) leukocyte cell surface antigens that cross-react with deer mouse cells. A panel of 19 monoclonal antibodies was screened for reactivity by flow cytometric analysis. Seven antibodies exhibited binding to deer mouse splenocytes at varying levels compared to house mouse controls. The panel detected cellular phenotypes that are associated with natural and adaptive immune responses, including T cells, B cells, macrophages, dendritic cells, neutrophils and granulocytes. This panel should facilitate the further examination of deer mouse immune responses to infectious agents.
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